Knihobot

Methods in Molecular Biology, Volume 155

Adipose Tissue Protocols

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  • 334 stránek
  • 12 hodin čtení

Více o knize

GA(c)rard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded to the isolation, subcellular fractionation, and transfection of low density adipocytes as well as metabolic aspects of nutrient uptake and assays of nutrient and ion fluxes. Innovative and highly practical, Adipose Tissue Protocols offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective research on adipose tissue from the nutritional to the physiological and the molecular levels.

Nákup knihy

Methods in Molecular Biology, Volume 155, Gérard Ailhaud

Jazyk
Rok vydání
2001
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Titul
Methods in Molecular Biology, Volume 155
Podtitul
Adipose Tissue Protocols
Jazyk
anglicky
Vydavatel
Humana Press
Rok vydání
2001
Vazba
pevná
Počet stran
334
ISBN10
0896037479
ISBN13
9780896037472
Série
Anotace
GA(c)rard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded to the isolation, subcellular fractionation, and transfection of low density adipocytes as well as metabolic aspects of nutrient uptake and assays of nutrient and ion fluxes. Innovative and highly practical, Adipose Tissue Protocols offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective research on adipose tissue from the nutritional to the physiological and the molecular levels.