Alberto Diaspro Knihy

The narrative explores the captivating journey of microscopy, highlighting the awe and discoveries it has brought to science. It intertwines themes of love, portraying a unique bond between glass and the spectrum of light, while presenting an original soundtrack that combines poetry and music. Through this artistic lens, the book celebrates the profound impact of microscopy on our understanding of the world.

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.