R. Ian Freshney Knihy

This new edition takes account of the progress that has been made in the field of animal cell biology since the first edition was published. The emphasis remains on presenting techniques in a readily accessible form, with detailed protocols given throughout.

This immensely useful interactive, laboratory-based reference and instructional guide to techniques involved in the culture of animal cells provides a link between textual information and visual demonstrations of techniques. Special features include more than 50 step-by-step protocols for cell culture, including basic and specialized techniques, supported by detailed textual and background information. Overviews summarize the protocols, link to text, video, and supplemental material, and facilitate navigation. Estimates are given for total procedure time and duration of each step. Extensive atlases of different cell types, culture stages, equipment photos, diagrams, tables, and graphs are included. Video sequences with narration illustrate 16 basic cell culture techniques. A compendium of recipes for commonly used reagents, a global directory of commercial suppliers, guidelines for setting up a cell culture laboratory, and advice on laboratory safety and biohazards are also provided.

Step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure. Written by leading experts in the field, Culture of Cells for Tissue Engineering offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures. Features of the text Culture of Cells for Tissue Engineering gives novice and seasoned researchers in tissue engineering an invaluable resource. In addition, the text is suitable for professionals in related research, particularly in those areas where cell and tissue culture is a new or emerging tool.