Practical enzymology

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This expanded third edition of a classic text on practical enzymology serves as a comprehensive guide, featuring new, lab-tested assays for over 30 enzymes across all classes, totaling 150 protocols for more than 110 enzymes. It introduces a section on determining enzyme-bound metal ions and addresses special cases such as multi-enzyme complexes, binding measurements, and immunoassays. The book is divided into two main parts: a general introduction to working with enzymes and a practical section with step-by-step protocols for enzyme assays, including methods for protein determination and enzyme immobilization. It covers various instrumental techniques, including spectroscopic and electrochemical methods, as well as radioactive labeling. The experimental section systematically details assays for oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases (synthetases). Later chapters delve into multienzyme complex assays, relevant enzyme parameters, and immunoassays. The text concludes with sections on binding measurements and technical applications of enzymes, including immobilization methods. With user-friendly formats and practical advice on chemical amounts and activity calculations, this edition is an essential resource for enzymologists.