Adult heart muscle cells

Více o knize

The content covers a range of topics related to isolated adult heart cells and their experimental models. It discusses the development of new models, including Ca-tolerant guinea-pig ventricular myocytes and guinea-pig atrial cardioballs, and methods for determining myocyte viability through staining and functional criteria. The importance of sarcolemma-bound calcium for cell viability and the effects of anoxic injury on cardiac myocytes are highlighted. Non-invasive measurements of cell surface receptors and the use of surface antigens in diagnosis are also examined. The substrate utilization and lactate transfer characteristics of adult cardiac myocytes are discussed, along with the insulin receptor's role in these cells. Further exploration includes the kinetics of cellular uptake of tracers for myocardial scintigraphy, adhesion of cardiac myocytes to extracellular matrix components, and the development of intercellular contacts in culture. Long-term primary cultures of human and rat cardiomyocytes reveal morphological dedifferentiation when cocultured with hepatocytes. Electrophysiological properties, such as the effects of cyanide on K-current and measurements of single-channel currents, are investigated. The correlation between catecholamine-induced damage and lipid peroxidation, as well as the influence of anoxia on glycolytic energy production and lipid probe diffusion, are also addressed. The document concludes with