Modern Methods in Protein Chemistry/Modern Methods in Protein Chemistry. Volume 3

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This comprehensive work covers various advanced techniques and methodologies in protein analysis and synthesis. It begins with strategies for localizing and synthesizing protein binding sites, followed by continuous flow peptide synthesis. The specificity of autoantibodies to small nuclear ribonucleoproteins in sera from patients with connective tissue diseases is explored, alongside new ultrasensitive detection systems for bioluminescence-enhanced enzyme immunoassays. Practical considerations in these assays are illustrated with a neutral proteinase inhibitor from porcine leukocytes. The text discusses a highly sensitive bioluminescence-enhanced detection system for protein blotting, the use of colloidal metal particles in this process, and amino-acid sequence analysis of proteins separated by gel electrophoresis. It also covers photoaffinity labeling, microcharacterization of phosphoserine-containing proteins, and the localization of autophosphorylation sites in skeletal muscle phosphorylase kinase. Additional topics include the construction of gas-phase sequencers, a novel solid support for gas-liquid microsequencing, and automated amino acid analysis via HPLC. The work concludes with discussions on microcalorimetry of protein-ligand interactions, the application of bifunctional reagents for topological investigations, and crystallographic studies on ribosomes, along with cell culture techniques for testing biologically ac