A mutation in porcine IGF2 influencing muscle mass & fat deposition

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Focusing on the principle of positional cloning, this book explores the investigation of a paternally imprinted QTL linked to muscle mass and fat deposition in pigs, specifically within the IGF2 region of SSC2. It details the generation and annotation of porcine sequences for the IGF2 and H19 genes, aiming to identify the causative mutation for this significant QTL. The work highlights the first identification of a Quantitative Trait Nucleotide and discusses its implications and applications in genetics and functional assays in the genomics era.