Detection, prevention and elimination of Mycoplasma contamination

Mycoplasma contamination poses a significant challenge in biomedical research, affecting 15-80% of cell cultures based on recent surveys. These slow-growing organisms can compromise experimental results by causing unreliable data and altering cellular functions without immediate destruction of the cultures. Effective detection methods such as IFA, Hoechst staining, and PCR are essential for identifying contamination before experiments begin. Preventing mycoplasma contamination is crucial not only for maintaining the integrity of cell cultures but also for ensuring the reliability of biological products.