Plant Protoplasts and Genetic Engineering II

Focusing on advanced techniques in agricultural biotechnology, this volume explores recent developments in genetic engineering, particularly through DNA recombinants and protoplast manipulation. It expands on earlier methods by introducing microinjections, electrofusion, and flow cytometry, detailing their roles in regenerating transgenic plants. The book reviews literature on genetic transformation methods, including Agrobacterium-mediated and direct gene transfer, and emphasizes the innovative uses of cytoplasts and miniprotoplasts in genetic manipulation of various crops and trees.