Laboruntersuchungen zur Entwicklung eines Desinfektionsmittelwirksamkeitsprüfungsverfahrens gegen Schadarthropoden mit exogenen Stadien der Roten Vogelmilbe Dermanyssus gallinae (De Geer 1778)

Disinfection is an indispensable component in the strategic control of parasites. In the Disinfectant Efficacy Testing, regarding parasitic field, only protozoa and helminths are considered to the DVG-guidelines (2000, 2007; from 2015 DVG-testing guidelines). Musca domestica can be used as an indicator in testing methods against harmful arthropods (Victor, 1999; Mielke et al, 2001; Simmet, 2013; Al Halbouni and Hiepe, 2015; Hiepe et al., 2015). The wide spectrum of arthropods species requires a representative for Acari as Parasites indicator, in addition to M. domestica as representative for insects. After extensive preliminary examinations, it has been found that Dermanyssus gallinae is suitable to be this indicator. That is because D. gallinae is able to represent harmful arthropod spectrum as an infectious agent as well as a vector. The problem of the low reproductive rate of these parasites (average 4-7 eggs / female / week) has been solved by the biostatistical method „one-sample t-test“. By Using D. gallinae laboratory strain H-0 another two problems have been solved: the exclusion of potential drug resistance of field strains and ensuring a stable D. gallinae- reproduction rate by the in vivo feeding. After intensive literature studies about disinfectant problems, with special consideration of antiparasitic disinfectants, a many examinations on chemical disinfectant efficacy testing, three produkts with different active ingredients groups have been selected: - Neopredisan 135-1; ingredient: p-chloro-m-cresol - Ascarosteril AB; ingredients in Component A: o -Hydroxydiphenyl fatty acid eutectic and in component B: peracetic acid. - Preventol VET 100; ingredients: 4-chloro-3-methyl phenol, 2-benzyl-4-chlorophenol, biphenyl-2-ol By Neopredisan 135-1 and Ascarosteril AB all D. gallinae-developmental stages (eggs, larvae, nymphs I and II) as well as hungry and full soaked adults have been included in the disinfectant efficacy testing. The examination of Preventol VET 100 was carried out exclusively on eggs of D. gallinae and soaked adults, as there were no significant differences in efficacy within the different stages of the parasite. The methodical equipment has been proved scientifically and technically through further development of germ carriers using poplar wood and concrete, and thus it fulfills the requirements of the DVG-testing standards and guidlines. The three tested disinfectants with the different drug components meet the target minimum efficiencies of DVG disinfectant commission (98% in suspension test, 95% in germ carrier tests) within the prescribed exposure times at 3% concentration. Eggs and full soaked adults are recommended for testing the effectiveness of a disinfectants against harmful arthropod D. gallinae eggs.